2026-04-13T05:59:25Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/2259432026-04-07T22:41:47Zcom_2072_1057col_2072_478781col_2072_478917

00925njm 22002777a 4500 dc Putra, Surya Rosa author Lois Rojas, Luisa María author Campos Martínez, Narciso author Boronat i Margosa, Albert author Rhomer, Michel author 2026-01-22T11:15:33Z 2026-01-22T11:15:33Z 1998-01-01 2026-01-22T11:15:33Z [2,3-13C2]- and [2,4-13C2]-d-1-deoxyxylulose were synthesized from [2-13C]pyruvate and [1-13C]- or [2-13C]-dl-glyceraldehyde using the enzyme d-1-deoxyxylulose 5-phosphate synthase from Escherichia coli which was overexpressed in this bacterium. These doubly-labeled isoprenoid precursors in the mevalonate independent route were incorporated into the ubiquinone of E. coli. 1J 13C/13C coupling constants were respectively found in isoprenic units between carbon atoms derived from C-3 and C-4 of isopentenyl diphosphate using the former labeled precursor or between C-3 and C-2 using the latter, indicating that d-1-deoxyxylulose was incorporated without prior degradation into isoprenoids and confirming that the branched isoprenic skeleton resulted from a rearrangement of the straight chain from carbohydrate precursor. © 1997 Elsevier Science Ltd. All rights reserved. Enzims microbians Escheríchia coli Química microbiològica Microbial enzymes Escherichia coli Microbiological chemistry Incorporation of [2,3-13C2]- and [2,4-13C 2]-D-1-deoxyxululose into ubiquinone of Escherichia coli via the mevalonate-independent pathway for isoprenoid biosynthesis