2026-04-14T03:27:07Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/2259352026-04-07T19:03:10Zcom_2072_1057col_2072_478781col_2072_478917

Identification of gcpE as a novel gene of the 2-C-methyl-D-erythritol 4-phosphate pathway for isoprenoid biosynthesis in Escherichia coli. Campos Martínez, Narciso Rodríguez Concepción, Manuel Seemann, Myriam Rohmer, Michel Boronat i Margosa, Albert Biologia molecular Associació molecular Molecular biology Molecular association The 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway for isoprenoid biosynthesis is essential in most eubacteria and plants and has remarkable biotechnological interest. However, only the first steps of this pathway have been determined. Using bioinformatic and genetic approaches, we have identified gcpE as a novel gene of the MEP pathway. The distribution of this gene in bacteria and plants strictly parallels that of the gene encoding 1-deoxy-D-xylulose 5-phosphate reductoisomerase, which catalyses the first committed step of the MEP pathway. Our data demonstrate that the gcpE gene is essential for the MEP pathway in Escherichia coli and indicate that this gene is required for the trunk line of the isoprenoid biosynthetic route. 2026-01-22T10:25:47Z 2026-01-22T10:25:47Z 2001-01-26 2026-01-22T10:25:47Z info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Versió postprint del document publicat a: https://doi.org/10.1016/S0014-5793(00)02420-0 FEBS Letters, 2001, vol. 488, num.3, p. 170-173 https://doi.org/10.1016/S0014-5793(00)02420-0 (c) Federation of European Biochemical Societies (FEBS), 2001 info:eu-repo/semantics/openAccess FEBS Press Articles publicats en revistes (Bioquímica i Biomedicina Molecular)