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oai:recercat.cat:2445/2258842026-04-07T19:02:35Zcom_2072_1057col_2072_478781col_2072_478917

Enhanced flux through the methylerythritol phosphate pathway in Arabidopsis plants overexpressing deoxyxylulose 5-phosphate reductoisomerase Carretero Paulet, Lorenzo Cairó Calzada, Albert Botella Pavía, Patricia Besumbes, Oscar Campos Martínez, Narciso Boronat i Margosa, Albert Rodríguez Concepción, Manuel Metabolisme de les plantes Biologia molecular vegetal Plant metabolism Plant molecular biology The methylerythritol 4-phosphate (MEP) pathway synthesizes the precursors for an astonishing diversity of plastid isoprenoids, including the major photosynthetic pigments chlorophylls and carotenoids. Since the identification of the first two enzymes of the pathway, deoxyxylulose 5-phoshate (DXP) synthase (DXS) and DXP reductoisomerase (DXR), they both were proposed as potential control points. Increased DXS activity has been shown to up-regulate the production of plastid isoprenoids in all systems tested, but the relative contribution of DXR to the supply of isoprenoid precursors is less clear. In this work, we have generated transgenic <em>Arabidopsis thaliana</em> plants with altered DXS and DXR enzyme levels, as estimated from their resistance to clomazone and fosmidomycin, respectively. The down-regulation of DXR resulted in variegation, reduced pigmentation and defects in chloroplast development, whereas DXR-overexpressing lines showed an increased accumulation of MEP-derived plastid isoprenoids such as chlorophylls, carotenoids, and taxadiene in transgenic plants engineered to produce this non-native isoprenoid. Changes in DXR levels in transgenic plants did not result in changes in DXS gene expression or enzyme accumulation, 2026-01-21T13:08:48Z 2026-01-21T13:08:48Z 2006-08-29 2026-01-21T13:08:48Z info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion 0167-4412 https://hdl.handle.net/2445/225884 592390 eng Versió postprint del document publicat a: https://doi.org/10.1007/s11103-006-9051-9 Plant Molecular Biology, 2006, vol. 62, p. 683-695 https://doi.org/10.1007/s11103-006-9051-9 (c) Springer Verlag, 2006 info:eu-repo/semantics/openAccess 13 p. application/pdf Springer Verlag Articles publicats en revistes (Bioquímica i Biomedicina Molecular)