2026-04-14T06:01:20Zhttps://recercat.cat/oai/request
oai:recercat.cat:2445/2253162026-04-07T18:54:52Zcom_2072_1057col_2072_478822col_2072_478914col_2072_478917
RECERCAT author Houben, Akke Mats author García Ojalvo, Jordi author Soriano i Fradera, Jordi 2026-01-12T16:16:10Z2026-01-12T16:16:10Z2025-11-062026-01-12T16:16:10Z An inherent challenge in designing laboratory-grown, engineered living neuronal networks lies in predicting the dynamic repertoire of the resulting network and its sensitivity to experimental variables. To fill this gap, and inspired by recent experimental studies, we present a numerical model designed to replicate the anisotropies in connectivity introduced through engineering, characterize the emergent collective behavior of the neuronal network, and make predictions. The numerical model is developed to replicate experimental data, and subsequently used to quantify network dynamics in relation to tunable structural and dynamical parameters. These include the strength of imprinted anisotropies, synaptic noise, and average axon lengths. We show that the model successfully captures the behavior of engineered neuronal cultures, revealing a rich repertoire of activity patterns that are highly sensitive to connectivity architecture and noise levels. Specifically, the imprinted anisotropies promote modularity and high clustering coefficients, substantially reducing the pathological-like bursting of standard neuronal cultures, whereas noise and axonal length influence the variability in dynamical states and activity propagation velocities. Moreover, connectivity anisotropies significantly enhance the ability to reconstruct structural connectivity from activity data, an aspect that is important to understand the structure–function relationship in neuronal networks. Our work provides a robust in silico framework to assist experimentalists in the design of in vitro neuronal systems and in anticipating their outcomes. This predictive capability is particularly valuable in developing reliable brain-on-a-chip platforms and in exploring fundamental aspects of neural computation, including input–output relationships and information coding. cc-by (c) Houben, A.M, et al., 2025 http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess Anisotropia Neurociències Xarxes neuronals (Neurobiologia) Anisotropy Neurosciences Neural networks (Neurobiology) Role of connectivity anisotropies in the dynamics of cultured neuronal networks info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion