2026-04-17T14:08:46Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/2222732025-12-05T00:57:52Zcom_2072_1057col_2072_478799col_2072_478916col_2072_478917

00925njm 22002777a 4500 dc Pinto Hernández, Paola author Fernández Sanjurjo, Manuel author Paget, Daan author Caravia, Xurde M. author Roiz Valle, David author Castilla Silgado, Juan author Diez Robles, Sergio author Coto Vilcapoma, Almudena author Fernández Vivero, David author Gama Pérez, Pau author García Roves, Pablo Miguel author López Otin, Carlos author Zierath, Juleen R. author Krook, Anna author Fernández García, Benjamin author Tomás Zapico, Cristina author Iglesias Gutierrez, Eduardo author 2025-07-16T08:57:30Z 2025-07-16T08:57:30Z 2025-05-23 2025-07-11T08:05:06Z Objective: The adaptive response to different models of regular exercise involves complex tissue crosstalk. Our aim was to explore the involvement of extracellular vesicle (EV) microRNAs (miRNAs) in this process, the secretory role of skeletal muscle and its functional metabolic interaction with the liver. Methods: Plasma EV miRNAs obtained from mice after 4-weeks of endurance or resistance training were sequenced. Subsequent experiments using trained genetically modified mouse models and in vitro approaches involving knock-down and electrostimulated cells, were conducted. Results: Resistance training increased the expression of a group of 11 miRNAs functionally divided into two clusters. Among them, miR-29a-3p emerges as a molecular mediator released in EVs by skeletal muscle, with a relevant role in adaptation to endurance training, by contributing to modulate the expression and secretion of other miRNAs associated with training and regulating processes related to substrate availability, transport, and metabolic use in skeletal muscle and liver. Conclusions: Our study suggests that miR-29a-3p is a training-induced molecular mediator in the response and adaptation to resistance training, possibly due to its regulatory role in energy metabolism in skeletal muscle in response to exercise. (c) 2025 The Author(s). Published by Elsevier GmbH. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). Fisiologia de l'exercici Micro RNAs Exercise physiology MicroRNAs Training-induced plasma miR-29a-3p is secreted by skeletal muscle and contributes to metabolic adaptations to resistance exercise in mice