2026-04-17T08:06:10Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/2216952025-12-04T22:43:58Zcom_2072_1057col_2072_478825col_2072_478917

00925njm 22002777a 4500 dc Ramos-Barbero, Maria Dolores author Gómez-Gómez, Clara author Vique, Gloria author Sala Comorera, Laura author Rodríguez-Rubio, Lorena author Muniesa Pérez, Ma. Teresa author 2025-06-20T13:48:30Z 2025-06-20T13:48:30Z 2024-10-03 2025-06-20T13:48:30Z The order Crassvirales, which includes the prototypical crAssphage (p-crAssphage), is predominantly associated with humans, rendering it the most abundant and widely distributed group of DNA phages in the human gut. The reported human specificity and wide global distribution of p-crAssphage makes it a promising human fecal marker. However, the specificity for the human gut as well as the geographical distribution around the globe of other members of the order Crassvirales remains unknown. To determine this, a recruitment analysis using 91 complete, non-redundant genomes of crAss-like phages in human and animal viromes revealed that only 13 crAss-like phages among the 91 phages analyzed were highly specific to humans, and p-crAssphage was not in this group. Investigations to elucidate whether any characteristic of the phages was responsible for their prevalence in humans showed that the 13 human crAss-like phages do not share a core genome. Phylogenomic analysis placed them in three independent families, indicating that within the Crassvirales group, human specificity is likely not a feature of a common ancestor but rather was introduced on separate/independent occasions in their evolutionary history. The 13 human crAss-like phages showed variable geographical distribution across human metagenomes worldwide, with some being more prevalent in certain countries than in others, but none being universally identified. The varied geographical distribution and the absence of a phylogenetic relationship among the human crAss-like phages are attributed to the emergence and dissemination of their bacterial host, the symbiotic human strains of Bacteroides, across various human populations occupying diverse ecological niches worldwide. Bacteriòfags Microbiota intestinal Biogeografia Bacteriophages Gastrointestinal microbiome Biogeography Recruitment of complete crAss-like phage genomes reveals their presence in chicken viromes, few human-specific phages, and lack of universal detection