2026-04-04T02:24:33Zhttps://recercat.cat/oai/request
oai:recercat.cat:2445/2192122025-12-05T00:38:25Zcom_2072_1057col_2072_478917col_2072_478933
RECERCAT author Abrahão Bueno Morais, Mariana author Nin Hill, Alba author Rovira i Virgili, Carme 2025-02-24T18:36:16Z2025-02-24T18:36:16Z2023-062025-02-24T18:36:16Z The enzymatic breakdown of carbohydrates plays a critical role in several biological events and enables the development of sustainable processes to obtain bioproducts and biofuels. In this scenario, the design of efficient inhibitors for glycosidases that can act as drug targets and the engineering of carbohydrate-active enzymes with tailored catalytic properties is of remarkable importance. To guide rational approaches, it is necessary to elucidate enzyme molecular mechanisms, in particular understanding how the microenvironment modulates the conformational space explored by the substrate. Computer simulations, especially those based on ab initio methods, have provided a suitable atomic description of carbohydrate conformations and catalytic reactions in several glycosidase families. In this review, we will focus on how the active-site topology (pocket or cleft) and mode of cleavage (endo or exo) can affect the catalytic mechanisms adopted by glycosidases, in particular the substrate conformations along the reaction coordinate. cc-by-nc-nd (c) Abrahão Bueno Morais, Mariana, et al., 2023 http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess Sucre Dinàmica molecular Enzims Sugar Molecular dynamics Enzymes Glycosidase mechanisms: Sugar conformations and reactivity in endo- and exo-acting enzymes info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion