2026-04-14T04:54:24Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/2180132025-12-04T21:12:37Zcom_2072_1057col_2072_478781col_2072_478917

00925njm 22002777a 4500 dc Moragas Garcia, Núria author Fernández Nogueira, Patricia author Recalde Percaz, Leire author Inman L., Jamie author López Plana, Anna author Bergholtz, Helga author Noguera Castells, Aleix author Burgo, Pedro J. del author Chen, Xieng author Sorlie, Therese author Gascón, Pere author Bragado Domingo, Paloma author Bissell, Mina author Carbó Carbó, Neus author Fuster, Gemma author 2025-01-27T14:52:53Z 2025-01-27T14:52:53Z 2024-08-13 2025-01-27T14:52:53Z A better understanding of ductal carcinoma in situ (DCIS) is urgently needed to identify these preinvasive lesions as distinct clinical entities. Semaphorin 3F (SEMA3F) is a soluble axonal guidance molecule, and its coreceptors Neuropilin 1 (NRP1) and NRP2 are strongly expressed in invasive epithelial BC cells. Methods: We utilized two cell line models to represent the progression from a healthy state to the mild-aggressive or ductal carcinoma in situ (DCIS) stage and, ultimately, to invasive cell lines. Additionally, we employed in vivo models and conducted analyses on patient databases to ensure the translational relevance of our results. Results: We revealed SEMA3F as a promoter of invasion during the DCIS-to-invasive ductal carcinoma transition in breast cancer (BC) through the action of NRP1 and NRP2. In epithelial cells, SEMA3F activates epithelialmesenchymal transition, whereas it promotes extracellular matrix degradation and basal membrane and myoepithelial cell layer breakdown. Conclusions: Together with our patient database data, these proof-of-concept results reveal new SEMA3F-mediated mechanisms occurring in the most common preinvasive BC lesion, DCIS, and represent potent and direct activation of its transition to invasion. Moreover, and of clinical and therapeutic relevance, the effects of SEMA3F can be blocked directly through its coreceptors, thus preventing invasion and keeping DCIS lesions in the preinvasive state. Càncer de mama Proteïnes de membrana Tumors Breast cancer Membrane proteins Tumors The SEMA3F-NRP1/NRP2 axis is a key factor in the acquisition of invasive traits in in situ breast ductal carcinoma