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SimpliPyTEM: An open-source Python library and app to simplify transmission electron microscopy and TEM image analysis Ing, Gabriel Stewart, Andrew Battaglia, Giuseppe Ruiz-Perez, Lorena Microscòpia electrònica de transmissió Microscòpia electrònica Transmission electron microscopy Electron microscopy Introducing SimpliPyTEM, a Python library and accompanying GUI that simplifies the post-acquisition evaluation of transmission electron microscopy (TEM) images, helping streamline the workflow. After an imaging session, a folder of image and/or video files, typically containing low contrast and large file size 32-bit images, can be quickly processed via SimpliPyTEM into high-quality, high-contrast.jpg images with suitably sized scale bars. The app can also generate HTML or PDF files containing the processed images for easy viewing and sharing. Additionally, SimpliPyTEM specifically focuses on <em>in situ</em> TEM videos, an emerging field of EM involving the study of dynamic processes whilst imaging. The package allows fast data processing into preview movies, averages, image series, or motion-corrected averages. The accompanying Python library offers many standard image processing methods, all simplified to a single command, plus a module to analyse particle morphology and population. This latter application is particularly useful for life sciences investigations. User-friendly tutorials and clear documentation are included to help guide users through the processing and analysis. We invite the EM community to contribute to and further develop this open-source package. 2024-11-29T16:44:16Z 2024-11-29T16:44:16Z 2023-10-05 2024-11-29T16:44:16Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Reproducció del document publicat a: https://doi.org/doi.org/10.1371/journal.pone.0285691 PLoS One, 2023, vol. 18, num.10, p. e0285691 https://doi.org/doi.org/10.1371/journal.pone.0285691 cc-by (c) Ing, G. et al., 2023 http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess Public Library of Science (PLoS) Articles publicats en revistes (Física Aplicada)