2026-04-17T02:32:25Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/2142002025-11-19T10:45:12Zcom_2072_1057col_2072_478902col_2072_478917

00925njm 22002777a 4500 dc Garreta, Elena author Moya Rull, Daniel author Marco, Andrés author Amato, Gaia author Ullate Agote, Asier author Tarantino, Carolina author Gallo, Maria author Esporrín Ubieto, David author Centeno, Alberto author Vilas Zornoza, Amaia author Mestre, Rafael author Kalil, Maria author Gorroñogoitia, Izar author Zaldua, Ane Miren author Sánchez, Samuel author Izquierdo Reyes, Laura author Fernández Santos, M. Eugenia author Prosper, Felipe author Montserrat, Núria author 2024-07-02T14:57:26Z 2024-07-02T14:57:26Z 2024-05-19 To date strategies aiming to modulate cell to extracellular matrix (ECM) interactions during organoid derivation remain largely unexplored. Here renal decellularized extracellular matrix (dECM) hydrogels are fabricated from porcine and human renal cortex as biomaterials to enrich cell-to-ECM crosstalk during the onset of kidney organoid differentiation from human pluripotent stem cells (hPSCs). Renal dECM-derived hydrogels are used in combination with hPSC-derived renal progenitor cells to define new approaches for 2D and 3D kidney organoid differentiation, demonstrating that in the presence of these biomaterials the resulting kidney organoids exhibit renal differentiation features, and the formation of an endogenous vascular component. Based on these observations, a new method to produce kidney organoids with vascular-like structures is achieved through the assembly of hPSC-derived endothelial-like organoids with kidney organoids in 3D. Major readouts of kidney differentiation and renal cell morphology are assessed exploiting these culture platforms as new models of nephrogenesis. Overall, this work shows that exploiting cell-to-ECM interactions during the onset of kidney differentiation from hPSCs facilitates and optimizes current approaches for kidney organoid derivation thereby increasing the utility of these unique culture cell platforms for personalized medicine. Angiogènesi Malalties del ronyó Cèl·lules mare Neovascularization Kidney diseases Stem cells Natural Hydrogels Support Kidney Organoid Generation and Promote in vitro Angiogenesis