2026-04-13T06:46:34Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/2141622025-12-05T12:27:26Zcom_2072_1057col_2072_478916col_2072_478917

00925njm 22002777a 4500 dc Asensio Puig, Laura author Andrés Pablo, Álvaro de author Khannous Lleiffe, Olfat author Ibáñez, Raquel author Acera Pérez, Amèlia author Sanjosé Llongueras, Silvia de author Gabaldón, Toni author Alemany, Laia author Bruni, Laia author Pavón Ribas, Miquel Àngel author 2024-07-02T13:01:18Z 2024-07-02T13:01:18Z 2024-05-24 2024-07-02T09:41:15Z Recent studies have revealed the impact of human papillomavirus (HPV) infections on the cervicovaginal microbiome; however, few have explored the utility of self-collected specimens (SCS) for microbiome detection, obtained using standardised methods for HPV testing. Here, we present a proof-of-concept analysis utilising Oxford Nanopore sequencing of the 16S rRNA gene in paired samples collected either by the patient using an Evalyn Brush or collected by a physician using liquid-based cytology (LBC). We found no significant differences in the alpha-diversity estimates between the SCS and LBC samples. Similarly, when analysing beta-diversity, we observed a close grouping of paired samples, indicating that both collection methods detected the same microbiome features. The identification of genera and Lactobacillus species in each sample allowed for their classification into community state types (CSTs). Notably, paired samples had the same CST, while HPV-positive and -negative samples belonged to distinct CSTs. As previously described in other studies, HPV-positive samples exhibited heightened bacterial diversity, reduced Lactobacillus abundance, and an increase in genera like Sneathia or Dialister. Altogether, this study showed comparable results between the SCS and LBC samples, underscoring the potential of self-sampling for analysing the microbiome composition in cervicovaginal samples initially collected for HPV testing in the context of cervical cancer screening. Papil·lomavirus Bacteriologia mèdica Papillomaviruses Medical bacteriology Proof of Concept Study: Comparability of Microbiome Diversity in Self- and Physician-Collected HPV-Positive and HPV-Negative Cervicovaginal Samples