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Generation of biological association networks: A novel strategy to detect new targets in cancer therapy Selga i Coma, Elisabet Almagro García, Ma. Cristina de Oleaga Sancho, Carlota Mencía Trinchant, Núria Ramírez, Sara Ruiz, F. Xavier Farrés i Vicén, Jaume Parés i Casasampera, Xavier Thibaut, Rémi Porte Visa, Cinta Noé Mata, Verónica Ciudad i Gómez, Carlos Julián Càncer Quimioteràpia Farmacogenètica Cancer Chemotherapy Pharmacogenetics Podeu consultar el llibre complet a: http://www.trnres.com/ebookcontents.php?id=149 The aim of this work was to design a novel strategy to detect new targets for anticancer treatments. The rationale was to build Biological Association Networks from differentially expressed genes in drug-resistant cells to identify important nodes within the Networks. These nodes may represent putative targets to attack in cancer therapy, as a way to destabilize the gene network developed by the resistant cells to escape from the drug pressure. As a model we used cells resistant to methotrexate (MTX), an inhibitor of DHFR. Selected node-genes were analyzed at the transcriptional level and from a genotypic point of view. In colon cancer cells, DHFR, the AKR1 family, PKC¿, S100A4, DKK1, and CAV1 were overexpressed while E-cadherin was lost. In breast cancer cells, the UGT1A family was overexpressed, whereas EEF1A1 was overexpressed in pancreatic cells. Interference RNAs directed against these targets sensitized cells towards MTX. 2012-01-09T09:18:20Z 2012-01-09T09:18:20Z 2011 2011-12-27T14:10:22Z info:eu-repo/semantics/bookPart info:eu-repo/semantics/publishedVersion https://hdl.handle.net/2445/21368 249207 eng Reproducció del capítol 1 del llibre publicat a: http://www.trnres.com/ebookcontents.php?id=106 Recent Advances in Pharmaceutical Sciences, 2011, Chapter 1, p. 1-33. Editor: Diego Muñoz-Torrero http://hdl.handle.net/2445/32392 (c) Transworld Research Network, 2011 info:eu-repo/semantics/openAccess 33 p. application/pdf Transworld Research Network Llibres / Capítols de llibre (Bioquímica i Biomedicina Molecular)