2026-04-13T05:36:24Zhttps://recercat.cat/oai/request
oai:recercat.cat:2445/2098222025-11-19T10:44:59Zcom_2072_1057col_2072_478902col_2072_478917
RECERCAT author Eills, James author Picazo Frutos, Román author Bondar, Oksana author Cavallari, Eleonora author Carrera, Carla author Barker, Sylwia J author Utz, Marcel author Herrero Gómez, Alba author Marco Rius, Irene author Tayler, Michael C. D. author Aime, Silvio author Reineri, Francesca author Budker, Dmitry author Blanchard, John W 2024-04-12T09:40:38Z2024-04-12T09:40:38Z2023-12-042024-01-31T12:44:07Z We demonstrate that enzyme-catalyzed reactions can be observed in zero- and low-field NMR experiments by combining recent advances in parahydrogen-based hyperpolarization methods with state-of-the-art magnetometry. Specifically, we investigated two model biological processes: the conversion of fumarate into malate, which is used in vivo as a marker of cell necrosis, and the conversion of pyruvate into lactate, which is the most widely studied metabolic process in hyperpolarization-enhanced imaging. In addition to this, we constructed a microfluidic zero-field NMR setup to perform experiments on microliter-scale samples of [1-C-13]-fumarate in a lab-on-a-chip device. Zero- to ultralow-field (ZULF) NMR has two key advantages over high-field NMR: the signals can pass through conductive materials (e.g., metals), and line broadening from sample heterogeneity is negligible. To date, the use of ZULF NMR for process monitoring has been limited to studying hydrogenation reactions. In this work, we demonstrate this emerging analytical technique for more general reaction monitoring and compare zero- vs low-field detection. (c) Eills, James et al, 2023 info:eu-repo/semantics/openAccess Ressonància magnètica Hidrogenació Magnetic resonance Hydrogenation Enzymatic Reactions Observed with Zero- and Low-Field Nuclear Magnetic Resonance info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion