2026-04-13T18:32:33Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/2073022025-12-05T14:31:35Zcom_2072_1057col_2072_478917col_2072_478923

Phosphorous pentoxide-free bioactive glass exhibits dose-dependent angiogenic and osteogenic capacities which are retained in glass polymeric composite scaffolds Font Tellado, Sonia Delgado, José Angel Poh, Su Ping Patrina Zhang, Wei Garcia Vallès, Maite Martínez Manent, Salvador Gorustovich, Alejandro Morejon Alonso, Lizette Van Griensven, Martijn Balmayor, Elizabeth Rosado Geoquímica Fòsfor Vidre Geochemistry Phosphorus Glass Bioactive glasses (BGs) are attractive materials for bone tissue engineering because of their bioactivity and</p><p>osteoinductivity. In this study, we report the synthesis of a novel phosphorous pentoxide-free, silicatebased</p><p>bioactive glass (52S-BG) composed of 52.1% SiO2, 23.2% Na2O and 22.6% CaO (wt%). The glass</p><p>was thoroughly characterized. The biocompatibility and osteogenic properties of 52S-BG particles were</p><p>analyzed in vitro with human adipose-derived mesenchymal stem cells (AdMSCs) and human osteoblasts.</p><p>52S-BG particles were biocompatible and induced mineralized matrix deposition and the expression of</p><p>osteogenic markers (RunX2, alkaline phosphatase, osteocalcin, osteopontin, collagen I) and the angiogenic</p><p>marker vascular endothelial growth factor (VEGF). Angiogenic properties were additionally</p><p>confirmed in a zebrafish embryo model. 52S-BG was added to poly-ε-caprolactone (PCL) to obtain a</p><p>composite with 10 wt% glass content. Composite PCL/52S-BG scaffolds were fabricated by additive manufacturing</p><p>and displayed high porosity (76%) and pore interconnectivity. The incorporation of 52S-BG particles</p><p>increased the Young’s modulus of PCL scaffolds from 180 to 230 MPa. AdMSC seeding efficiency</p><p>and proliferation were higher in PCL/52S-BG compared to PCL scaffolds, indicating improved biocompatibility.</p><p>Finally, 52S-BG incorporation improved the scaffolds’ osteogenic and angiogenic properties by</p><p>increasing mineral deposition and inducing relevant gene expression and VEGF protein secretion. Overall,</p><p>52S-BG particles and PCL/52S-BG composites may be attractive for diverse bone engineering applications</p><p>requiring concomitant angiogenic properties. 2024-02-08T11:58:00Z 2024-02-08T11:58:00Z 2021-12-07 2024-02-08T11:58:01Z info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion 2047-4830 https://hdl.handle.net/2445/207302 716698 eng Versió postprint del document publicat a: https://doi.org/10.1039/d1bm01311d Biomaterials Science, 2021, vol. 9, num.23, p. 7876-7894 https://doi.org/10.1039/d1bm01311d (c) Font Tellado, S. et al., 2021 info:eu-repo/semantics/openAccess 45 p. application/pdf Royal Society of Chemistry Articles publicats en revistes (Mineralogia, Petrologia i Geologia Aplicada)