2026-04-17T06:34:34Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/2036232025-12-05T14:16:41Zcom_2072_1057col_2072_478902col_2072_478917

00925njm 22002777a 4500 dc Zabalo, Jon author Abril, Eduardo author Carré, Albane author García Díaz, María author Martinez, Elena author Torras Andrés, Núria author 2023-11-13T09:24:49Z 2023-11-13T09:24:49Z 2023-10-01 2023-10-06T06:50:55Z The intestine is a complex tissue with a characteristic three-dimensional (3D) crypt-villus architecture, which plays a key role in the intestinal function. This function is also regulated by the intestinal stroma that actively supports the intestinal epithelium, maintaining the homeostasis of the tissue. Efforts to account for the 3D complex structure of the intestinal tissue have been focused mainly in mimicking the epithelial barrier, while solutions to include the stromal compartment are scarce and unpractical to be used in routine experiments. Here we demonstrate that by employing an optimized bioink formulation and the suitable printing parameters it is possible to produce fibroblast-laden crypt-villus structures by means of digital light projection stereolithography (DLP-SLA). This process provides excellent cell viability, accurate spatial resolution, and high printing throughput, resulting in a robust biofabrication approach that yields functional gut mucosa tissues compatible with conventional testing techniques.Copyright © 2023 Elsevier B.V. All rights reserved. Impressió 3D Teixits (Histologia) Three-dimensional printing Tissues A bioprinted 3D gut model with crypt-villus structures to mimic the intestinal epithelial-stromal microenvironment