2026-04-17T03:01:59Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/1985932025-12-04T22:47:19Zcom_2072_1057col_2072_478825col_2072_478917

urn:hdl:2445/198593 Structural diversity among Edwarsiellaceae core oligosaccharides. Jordán, Maria Wojtys-Tekiel, Sylwia Merino Montero, Susana Tomàs Magaña, Juan Kaszowska, Marta Bacteris gramnegatius Genòmica Oligosacàrids Espectroscòpia de ressonància magnètica nuclear Gram-negative bacteria Genomics Oligosaccharides Nuclear magnetic resonance spectroscopy The Edwardsiella genus presents five different pathogenic species: Edwardsiella tarda, E. anguillarum, E. piscicida, E. hoshinae and E. ictaluri. These species cause infections mainly in fish, but they can also infect reptiles, birds or humans. Lipopolysaccharide (endotoxin) plays an important role in the pathogenesis of these bacteria. For the first time, the chemical structure and genomics of the lipopolysaccharide (LPS) core oligosaccharides of E. piscicida, E. anguillarum, E. hoshinae and E. ictaluri were studied. The complete gene assignments for all core biosynthesis gene functions were acquired. The structure of core oligosaccharides was investigated by 1H and 13C nuclear magnetic resonance (NMR) spectroscopy. The structures of E. piscicida and E. anguillarum core oligosaccharides show the presence of !3,4)-L-glycero- -D-manno-Hepp, two terminal -D-Glcp, !2,3,7)-L-glycero- -Dmanno- Hepp,!7)-L-glycero- -D-manno-Hepp, terminal -D-GlcpN, two!4)- -D-GalpA,! 3)- -DGlcpNAc, terminal -D-Galp and !5-substituted Kdo. E. hoshinare core oligosaccharide shows only one terminal -D-Glcp, and instead of terminal -D-Galp a terminal -D-GlcpNAc. E. ictaluri core oligosaccharide shows only one terminal -D-Glcp, one !4)- -D-GalpA and do not have terminal -D-GlcpN (see complementary figure). 2023-05-30T12:36:24Z 2023-05-30T12:36:24Z 2023-03-01 2023-05-30T12:36:24Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Reproducció del document publicat a: https://doi.org/10.3390/ijms24054768 International Journal of Molecular Sciences, 2023, vol. 24, num. 5, p. 4768 https://doi.org/10.3390/ijms24054768 cc-by (c) Jordán, Maria et al., 2023 https://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess MDPI Articles publicats en revistes (Genètica, Microbiologia i Estadística)