2026-04-19T17:46:38Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/1972832025-12-04T21:28:19Zcom_2072_1057col_2072_478812col_2072_478902col_2072_478917

00925njm 22002777a 4500 dc Casanellas, Ignasi author Jiang, Hongkai author David, Carolyn M. author Vida, Yolanda author Pérez Inestrosa, Ezequiel author Samitier i Martí, Josep author Lagunas, Anna author 2023-04-26T11:57:51Z 2023-04-26T11:57:51Z 2022-11-01 2023-04-26T11:57:51Z Mesenchymal condensation is a prevalent morphogenetic transition that is essential in chondrogenesis. However, the current understanding of condensation mechanisms is limited. In vivo, progenitor cells directionally migrate from the surrounding loose mesenchyme towards regions of increasing matrix adherence (the condensation centers), which is accompanied by the upregulation of fibronectin. Here, we focused on the mechanisms of cell migration during mesenchymal cell condensation and the effects of matrix adherence. Dendrimer-based nanopatterns of the cell-adhesive peptide arginine-glycine-aspartic acid (RGD), which is present in fibronectin, were used to regulate substrate adhesion. We recorded collective and single-cell migration of mesenchymal stem cells, under chondrogenic induction, using live-cell imaging. Our results show that the cell migration mode of single cells depends on substrate adhesiveness, and that cell directionality controls cell condensation and the fusion of condensates. Inhibition experiments revealed that cell-cell interactions mediated by N-cadherin (also known as CDH2) are also pivotal for directional migration of cell condensates by maintaining cell-cell cohesion, thus suggesting a fine interplay between cell-matrix and cell-cell adhesions. Our results shed light on the role of cell interactions with a fibronectin-depositing matrix during chondrogenesis in vitro, with possible applications in regenerative medicine. This article has an associated First Person interview with the first author of the paper. Migració cel·lular Condrogènesi Materials nanoestructurats Cell migration Chondrogenesis Nanostructured materials Substrate adhesion determines migration during mesenchymal cell condensation in chondrogenesis