2026-04-13T06:49:33Zhttps://recercat.cat/oai/requestoai:recercat.cat:2445/1937152025-12-04T20:44:16Zcom_2072_1057col_2072_478822col_2072_478917
RECERCAT
author
Estévez Priego, Estefanía
author
Moreno Fina, Martina
author
Monni, Emanuela
author
Kokaia, Zaal
author
Soriano i Fradera, Jordi
author
Tornero, Daniel
2023-02-17T11:09:30Z2023-02-17T11:09:30Z2023-01-102023-02-17T11:09:30Z
Models for human brain-oriented research are often established on primary cultures from rodents, which fails to recapitulate cellular specificity and molecular cues of the human brain. Here we investigated whether neuronal cultures derived from human induced pluripotent stem cells (hiPSCs) feature key advantages compared with rodent primary cultures. Using calcium fluorescence imaging, we tracked spontaneous neuronal activity in hiPSC-derived, human, and rat primary cultures and compared their dynamic and functional behavior as they matured.We observed that hiPSC-derived cultures progressively changed upon development, exhibiting gradually richer activity patterns and functional traits. By contrast, rat primary cultures were locked in the same dynamic state since activity onset. Human primary cultures exhibited features in between hiPSC-derived and rat primary cultures, although traits from the former predominated. Our study demonstrates that hiPSC-derived cultures are excellent models to investigate development in neuronal assemblies, a hallmark for applications that monitor alterations caused by damage or neurodegeneration.
cc-by (c) Estévez Priego, Estefanía et al., 2023 https://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess
Cèl·lules mare
Xarxes neuronals (Neurobiologia)
Stem cells
Neural networks (Neurobiology)
Long-term calcium imaging reveals functional development in hiPSC-derived cultures comparable to human but not rat primary cultures
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion