2026-04-13T03:09:18Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/1937152025-12-04T20:44:16Zcom_2072_1057col_2072_478822col_2072_478917

00925njm 22002777a 4500 dc Estévez Priego, Estefanía author Moreno Fina, Martina author Monni, Emanuela author Kokaia, Zaal author Soriano i Fradera, Jordi author Tornero, Daniel author 2023-02-17T11:09:30Z 2023-02-17T11:09:30Z 2023-01-10 2023-02-17T11:09:30Z Models for human brain-oriented research are often established on primary cultures from rodents, which fails to recapitulate cellular specificity and molecular cues of the human brain. Here we investigated whether neuronal cultures derived from human induced pluripotent stem cells (hiPSCs) feature key advantages compared with rodent primary cultures. Using calcium fluorescence imaging, we tracked spontaneous neuronal activity in hiPSC-derived, human, and rat primary cultures and compared their dynamic and functional behavior as they matured.We observed that hiPSC-derived cultures progressively changed upon development, exhibiting gradually richer activity patterns and functional traits. By contrast, rat primary cultures were locked in the same dynamic state since activity onset. Human primary cultures exhibited features in between hiPSC-derived and rat primary cultures, although traits from the former predominated. Our study demonstrates that hiPSC-derived cultures are excellent models to investigate development in neuronal assemblies, a hallmark for applications that monitor alterations caused by damage or neurodegeneration. Cèl·lules mare Xarxes neuronals (Neurobiologia) Stem cells Neural networks (Neurobiology) Long-term calcium imaging reveals functional development in hiPSC-derived cultures comparable to human but not rat primary cultures