2026-04-17T05:49:50Zhttps://recercat.cat/oai/request
oai:recercat.cat:2445/1934872025-12-04T21:12:57Zcom_2072_1057col_2072_478781col_2072_478917
RECERCAT author Domingo, Pere author Quesada López, Tania Paloma author Villarroya i Terrade, Joan author Cairó Calzada, Montserrat author Gutierrez, Maria Del Mar author Mateo, Maria Gracia author Mur, Isabel author Corbacho, Noemí author Domingo i Pedrol, Joan Carles author Villarroya i Gombau, Francesc author Giralt i Oms, Marta 2023-02-13T09:44:17Z2023-02-13T09:44:17Z2022-11-012023-02-13T09:44:17Z Aims: To assess the potential direct effects of the integrase strand-transfer inhibitors (INsTIs) dolutegravir, bictegravir, and raltegravir, drugs used as treatment for people living with human immunodeficiency virus (PLWH), on human adipose cells. Main methods: Drugs were added to the differentiation medium of human Simpson-Golabi-Behmel syndrome (SGBS) adipose cells and morphological adipogenesis was monitored for 10 days. Also, adipocytes were exposed to drugs following differentiation (day 14). The gene expression levels of selected adipogenesis markers, adipocyte metabolism markers, adipokines, and cytokines were determined by quantitative-reverse transcription polymerase-chain reaction. The release of adiponectin and leptin into the culture medium was measured using specific enzyme-linked immunosorbent assay, and release of interleukin-6 and chemokine (CC motif) ligand-2 using Multiplex assays. Key findings: Overall morphological adipogenesis was unaltered by INsTIs. The expression of adipogenesis marker genes (peroxisome proliferator-activated receptor-Ɣ and lipoprotein lipase) was slightly reduced in dolutegravir-treated differentiating adipocytes. Bictegravir repressed gene expression and the release of pro-inflammatory cytokines in differentiating adipocytes. Dolutegravir and raltegravir increased interleukin-6 gene expression, but only dolutegravir increased interleukin-6 release. Dolutegravir repressed adiponectin expression and release in differentiating adipocytes and had a similar but milder effect on leptin. Drug treatment of mature adipocytes reduced adiponectin gene expression in response to dolutegravir. Significance: The INsTIs studied do not have a significant effect on human adipose cell differentiation but exert distinct effects on gene expression and secretion of adipokines and cytokines. These findings will help understand and manage the effects of INsTI-containing treatments on body weight and metabolic dysregulation in PLWH. cc-by-nc-nd (c) Domingo, Pere et al., 2022 https://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess Citoquines Immunitat cel·lular Vitamines liposolubles Cytokines Cellular immunity Fat-soluble vitamins Differential effects of dolutegravir, bictegravir and raltegravir in adipokines and inflammation markers on human adipocytes. info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion