2026-04-13T18:25:42Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/1934822025-12-04T22:42:54Zcom_2072_1057col_2072_478825col_2072_478917

urn:hdl:2445/193482 Monitoring emergence of SARS-CoV-2 B.1.1.7 Variant through the Spanish National SARSCoV-2 Wastewater Surveillance System (VATar COVID-19) from December 2020 to March 2021 Carcereny, Albert Martínez-Velázquez, Adán Bosch, Albert Allende, Anna Truchado, Pilar Cascales, Jenifer Romalde, Jesús Lois, Marta Polo, David Sánchez, Gloria Pérez-Cataluña, Alba Díaz-Reolid, Azahara Antón, Andrés Gregor, Josep Garcia-Cehic, Damir Quer, Josep Palau, Margarita González Ruano, Cristina Pintó Solé, Rosa María Guix, Susana SARS-CoV-2 COVID-19 Epidemiologia SARS-CoV-2 COVID-19 Epidemiology Since its first identification in the United Kingdom in late 2020, the highly transmissible B.1.1.7 variant of SARS-CoV-2 has become dominant in several countries raising great concern. We developed a duplex real-time RT-qPCR assay to detect, discriminate, and quantitate SARS-CoV-2 variants containing one of its mutation signatures, the ΔHV69/70 deletion, and used it to trace the community circulation of the B.1.1.7 variant in Spain through the Spanish National SARS-CoV-2 Wastewater Surveillance System (VATar COVID-19). The B.1.1.7 variant was detected earlier than clinical epidemiological reporting by the local authorities, first in the southern city of Málaga (Andalucía) in week 20_52 (year_week), and multiple introductions during Christmas holidays were inferred in different parts of the country. Wastewater-based B.1.1.7 tracking showed a good correlation with clinical data and provided information at the local level. Data from wastewater treatment plants, which reached B.1.1.7 prevalences higher than 90% for ≥2 consecutive weeks showed that 8.1 ± 2.0 weeks were required for B.1.1.7 to become dominant. The study highlights the applicability of RT-qPCR-based strategies to track specific mutations of variants of concern as soon as they are identified by clinical sequencing and their integration into existing wastewater surveillance programs, as a cost-effective approach to complement clinical testing during the COVID-19 pandemic. 2023-02-13T08:55:33Z 2023-02-13T08:55:33Z 2021 2023-02-13T08:55:33Z info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Versió postprint del document publicat a: https://doi.org/10.1021/acs.est.1c03589 Environmental Science & Technology, 2021, vol. 55, num. 17, p. 11756-11766 https://doi.org/10.1021/acs.est.1c03589 (c) American Chemical Society , 2021 info:eu-repo/semantics/openAccess American Chemical Society Articles publicats en revistes (Genètica, Microbiologia i Estadística)