2026-04-17T05:57:20Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/1912952025-12-05T09:35:26Zcom_2072_1057col_2072_478781col_2072_478799col_2072_478908col_2072_478917

00925njm 22002777a 4500 dc Errasti-Murugarren, Ekaitz author Bartoccioni, Paola author Palacín Prieto, Manuel author 2022-12-01T15:55:50Z 2022-12-01T15:55:50Z 2021-02-22 2022-12-01T15:55:50Z Accounting for nearly two-thirds of known druggable targets, membrane proteins are highly relevant for cell physiology and pharmacology. In this regard, the structural determination of pharmacologically relevant targets would facilitate the intelligent design of new drugs. The structural biology of membrane proteins is a field experiencing significant growth as a result of the development of new strategies for structure determination. However, membrane protein preparation for structural studies continues to be a limiting step in many cases due to the inherent instability of these molecules in non-native membrane environments. This review describes the approaches that have been developed to improve membrane protein stability. Membrane protein mutagenesis, detergent selection, lipid membrane mimics, antibodies, and ligands are described in this review as approaches to facilitate the production of purified and stable membrane proteins of interest for structural and functional studies. Proteïnes de membrana Mutagènesi Detergents Lípids Membrane proteins Mutagenesis Detergents Lipids Membrane protein stabilization strategies for structural and functional studies