2026-04-17T01:39:30Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/1899422025-12-04T19:08:00Zcom_2072_1057col_2072_478858col_2072_478917col_2072_478924

00925njm 22002777a 4500 dc Laveriano-Santos, Emily P. author Marhuenda-Muñoz, María author Vallverdú i Queralt, Anna author Martínez Huélamo, Miriam author Tresserra i Rimbau, Anna author Miliarakis, Eleftherios author Arancibia Riveros, Camila author Jáuregui Pallarés, Olga author Ruíz León, Ana María author Castro-Barquero, Sara author Estruch, Ramon author Bodega, Patricia author Miguel, Mercedes de author Cos Gandoy, Amaya de author Martínez Gomez, Jesús author Santos-Beneit, Gloria author Fernández Alvira, Juan Miguel author Fernández Jiménez, Rodrigo author Lamuela Raventós, Rosa Ma. author 2022-10-17T08:21:17Z 2022-10-17T08:21:17Z 2022-06 2022-10-17T08:21:17Z This study aimed to develop and validate a liquid chromatography/electrospray ionization-linear ion trap quadrupole-Orbitrap-high-resolution mass spectrometry (HPLC/ESI-LTQ-Orbitrap-HRMS) method to identify and quantify urinary microbial phenolic metabolites (MPM), as well as to explore the relationship between MPM and dietary (poly)phenols in Spanish adolescents. A total of 601 spot urine samples of adolescents aged 12.02 ± 0.41 years were analyzed. The quantitative method was validated for linearity, limit of detection, limit of quantification, recovery, intra-and inter-day accuracy and precision, as well as postpreparative stability according to the criteria established by the Association of Official Agricultural Chemists International. A total of 17 aglycones and 37 phase II MPM were identified and quantified in 601 spot urine samples. Phenolic acids were the most abundant urinary MPM, whereas stilbenes, hydroxytyrosol, and enterodiol were the least abundant. Urinary hydroxycoumarin acids (urolithins) were positively correlated with flavonoid and total (poly)phenol intake. An HPLC-ESI-LTQ-Orbitrap-HRMS method was developed and fully validated to quantify MPM. The new method was performed accurately and is suitable for MPM quantification in large epidemiological studies. Urinary lignans and urolithins are proposed as potential biomarkers of grain and nut intake in an adolescent population. Microbiota intestinal Polifenols Adolescents Gastrointestinal microbiome Polyphenols Teenagers Identification and Quantification of Urinary Microbial Phenolic Metabolites by HPLC-ESI-LTQ-Orbitrap-HRMS and Their Relationship with Dietary Polyphenols in Adolescents