2026-04-14T03:30:40Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/1896932025-11-19T19:01:07Zcom_2072_1057col_2072_478777col_2072_478781col_2072_478902col_2072_478917

00925njm 22002777a 4500 dc Matera, Carlo author Calvé, Pablo author Casadó Anguera, Verònica author Sortino, Rosalba author Gomila, Alexandre M.J. author Moreno Guillén, Estefanía author Gener, Thomas author Delgado-Sallent, Cristina author Nebot, Pau author Costazza, Davide author Conde-Berriozábal, Sara author Masana Nadal, Mercè author Hernando, Jordi author Casadó, Vicent author Puig, M. Victoria author Gorostiza Langa, Pablo Ignacio author 2022-10-06T17:37:04Z 2022-10-06T17:37:04Z 2022-09-04 2022-10-06T17:37:04Z Understanding the dopaminergic system is a priority in neurobiology and neuropharmacology. Dopamine receptors are involved in the modulation of fundamental physiological functions and dysregulation of dopaminergic transmission is associated with major neurological disorders. However, the available tools to dissect the endogenous dopaminergic circuits have limited specificity, reversibility, resolution, or require genetic manipulation. Here we introduce azodopa, a novel photoswitchable ligand that enables reversible spatiotemporal control of dopaminergic transmission. We demonstrate that azodopa activates D1-like receptors in vitro in a light-dependent manner. Moreover, it enables reversibly photocontrolling zebrafish motility on a time scale of seconds and allows separating the retinal component of dopaminergic neurotransmission. Azodopa increases the overall neural activity in the cortex of anesthetized mice and displays illuminationdependent activity in individual cells. Azodopa is the first photoswitchable dopamine agonist with demonstrated efficacy in wildtype animals and opens the way to remotely controlling dopaminergic neurotransmission for fundamental and therapeutic purposes. Dopamina Electrofisiologia Dopamine Electrophysiology Reversible photocontrol of dopaminergic transmission in wild-type animals