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Disparate miRNA expression in serum and plasma of patients with acute myocardial infarction: a systematic and paired comparative analysis Mompeon, Ana Ortega Paz, Luis Vidal Gomez, Xavier Januario Costa, Tiago Perez Cremades, Daniel Garcia Blas, Sergio Brugaletta, Salvatore Sanchis Forés, Juan Sabaté Tenas, Manuel Novella, Susana Dantas, Ana Paula Hermenegildo, Carlos Vellesa Micro RNAs Infart de miocardi Plasma sanguini Old age MicroRNAs Myocardial infarction Blood plasma Despite the promising value of miRNAs in the diagnostic and prognostic of cardiovascular disease (CVD), recent meta-analyses did not support their potential. Methodological variances in studies may interfere with miRNA profle and afect their results. This study determines if the blood starting material is a source of variance in miRNA profle by performing a paired comparison in plasma and serum of the expression of primary miRNAs associated with CVD. Circulating miRNA yield was similar in both plasma and serum, although a signifcant increase was observed in patients with Non-ST-elevation myocardial infarction (NSTEMI) compared to control volunteers. When normalized by the expression of miR-484, diferent patterns of miRNA expression between serum and plasma. Although NSTEMI modifed the expression of miR-1 and miR-208 in both serum and plasma, plasma displayed a higher variance than serum (Levene's test p<0.01). For miR-133a and miR-26a, diferences were only detected in serum (p=0.0240), and conversely, miR-499a showed diferences only in plasma of NSTEMI (p=0.001). Interestingly, miR-21 showed an opposite pattern of expression, being increased in serum (2−ΔΔCt : 5.7, p=0.0221) and decreased in plasma (2−ΔΔCt : 0.5, p=0.0107). Plasma and serum exhibit diferent patterns of circulating miRNA expression in NSTEMI and suggest that results from studies with diferent starting material could not be comparable. 2022-06-22T15:31:13Z 2022-06-22T15:31:13Z 2020-03-25 2022-06-22T15:31:14Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Reproducció del document publicat a: https://doi.org/10.1038/s41598-020-61507-z Scientific Reports, 2020, vol. 10, num. 1, p. 5373 https://doi.org/10.1038/s41598-020-61507-z cc-by (c) Mompeon, Ana et al., 2020 https://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess Nature Publishing Group Articles publicats en revistes (Medicina)