2026-04-14T02:26:59Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/1862482026-02-12T19:14:08Zcom_2072_1057col_2072_478908col_2072_478917

00925njm 22002777a 4500 dc Bielskutė, Stasė author Garcia Cabau, Carla author Frigolé Vivas, Marta author Szulc, Elzbieta author Mol, Eva de author Pesarrodona, Mireia author Garcia, Jesús author Salvatella i Giralt, Xavier author 2022-06-02T08:49:51Z 2022-06-02T08:49:51Z 2021-05-12 2022-06-02T07:17:58Z The phase equilibria of intrinsically disordered proteins are exquisitely sensitive to changes in solution conditions and this can be used to investigate the driving forces of phase separation in vitro as well as the biological roles of phase transitions in live cells. Here we investigate how using D2O as co-solvent in an aqueous buffer changes the phase equilibrium of a fragment of the activation domain of the androgen receptor, a transcription factor that plays a role in the development of the male phenotype and is a therapeutic target for castration resistant prostate cancer. We show how replacing even small fractions of H2O with D2O increases the propensity of this fragment to undergo liquid-liquid phase separation, likely reflecting a stabilization of the hydrophobic interactions that drive condensation. Our results indicate that it is necessary to take this effect into consideration when studying phase separation phenomena with biophysical methods that require using D2O as a co-solvent. In addition, they suggest that additions of D2O may be used to enhance phase separation phenomena in cells, facilitating their observation. Receptors d'hormones Proteïnes Hormone receptors Proteins Low amounts of heavy water increase the phase separation propensity of a fragment of the androgen receptor activation domain