2026-04-04T02:27:20Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/1862392025-12-16T20:06:03Zcom_2072_1057col_2072_478781col_2072_478917

00925njm 22002777a 4500 dc Vallejo-Gracia, Albert author Sastre Martinez, Daniel author Colomer-Molera, Magalí author Solé, Laura author Navarro-Pérez, María author Capera Aragonés, Jesusa author Roig, Sara R. author Pedrós-Gámez, Oriol author Estadella, Irene author Szilágyi, Orsolya author Panyi, Gyorgy author Hajdú, Péter author Felipe Campo, Antonio author 2022-06-01T16:43:21Z 2022-06-01T16:43:21Z 2021-07-16 2022-06-01T16:43:22Z The voltage-dependent potassium channel Kv1.3 plays essential roles in the immune system, participating in leukocyte activation, proliferation and apoptosis. The regulatory subunit KCNE4 acts as an ancillary peptide of Kv1.3, modulates K+ currents and controls channel abundance at the cell surface. KCNE4-dependent regulation of the oligomeric complex fne-tunes the physiological role of Kv1.3. Thus, KCNE4 is crucial for Ca2+-dependent Kv1.3-related leukocyte functions. To better understand the role of KCNE4 in the regulation of the immune system, we manipulated its expression in various leukocyte cell lines. Jurkat T lymphocytes exhibit low KCNE4 levels, whereas CY15 dendritic cells, a model of professional antigen-presenting cells, robustly express KCNE4. When the cellular KCNE4 abundance was increased in T cells, the interaction between KCNE4 and Kv1.3 afected important T cell physiological features, such as channel rearrangement in the immunological synapse, cell growth, apoptosis and activation, as indicated by decreased IL-2 production. Conversely, ablation of KCNE4 in dendritic cells augmented proliferation. Furthermore, the LPS-dependent activation of CY15 cells, which induced Kv1.3 but not KCNE4, increased the Kv1.3-KCNE4 ratio and increased the expression of free Kv1.3 without KCNE4 interaction. Our results demonstrate that KCNE4 is a pivotal regulator of the Kv1.3 channelosome, which fne-tunes immune system physiology by modulating Kv1.3-associated leukocyte functions. Canals de potassi Sistema immunitari Leucòcits Cèl·lules T Potassium channels Immune system Leucocytes T cells KCNE4-dependent functional consequences of Kv1.3-related leukocyte physiology.