2026-04-13T00:52:07Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/1848632025-11-19T19:23:33Zcom_2072_1057col_2072_478813col_2072_478913col_2072_478917

00925njm 22002777a 4500 dc Mancera Arteu, Montserrat author Giménez López, Estela author Sancho, Jaime author Sanz Nebot, María Victoria author 2022-04-08T11:50:31Z 2022-04-08T11:50:31Z 2020-03-12 2022-04-08T11:50:31Z Transferrin purification from mice serum samples by immunoaffinity chromatography (IAC) was optimized in order to study the possible modifications occurred in its glycans in collagen-induced arthritis (CIA) samples. SDS-PAGE and nanoLC-MS/MS were used to monitor the IAC purification performance. Afterwards, a relative quantification of mouse transferrin (mTf) glycan isomers using [12C6]/[13C6]-aniline was used to unequivocally detect alterations in the glycan profile of CIA mice. In addition, multivariate data analysis was applied to identify the most meaningful glycan isomers for the discrimination between control and pathological samples. Partial least squares discriminant analysis (PLS-DA) revealed that five out of fifteen mTf glycan isomers could be potential biomarkers of CIA, most of them corresponding to highly sialylated structures (H6N5S3_2, H6N5S3_3 and H5N4S3_2). Moreover, some of these glycan isomers also seemed to be related with the progression of CIA, especially H6N5S2 and H6N5S3_2, as their overexpression increased with the clinical score of the pathology. Hence, the established methodology provides valuable information to find glycan-based biomarkers of CIA, but also leaves the door open to evaluate, in the future, glycosylation changes of many other inflammatory diseases, in which transferrin has been described to be altered. Artritis Glicoproteïnes Marcadors bioquímics Cromatografia d'afinitat Arthritis Glycoproteins Biochemical markers Affinity chromatography Alterations in the glycan profile of mouse transferrin: new insights in collagen-​induced arthritis