2026-04-17T05:49:33Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/1844972025-12-04T21:15:50Zcom_2072_1057col_2072_478781col_2072_478917

00925njm 22002777a 4500 dc Mleczko, J.E. author Royo, F. author Samuelson, I. author Clos-Garcia, M. author Williams, C. author Cabrera, D. author Azparren-Angulo, M. author Gonzalez, E. author Garcia-Vallicrosa, C. author Carobbio, S. author Rodriguez-Cuenca, S. author Azkargorta, M. author van Liempd, S. author Elortza, F. author Vidal-Puig, A. author Mora Fayos, Sílvia author Falcon-Perez, J.M. author 2022-03-29T11:59:45Z 2022-03-29T11:59:45Z 2022-03-15 2022-03-29T11:59:45Z The composition of extracellular vesicles (EVs) is altered in many pathological conditions, and their molecular content provides essential information on features of parent cells and mechanisms of crosstalk between cells and organs. Metabolic Syndrome (MetS) is a cluster of clinical manifestations including obesity, insulin resistance, dyslipidemia and hypertension that increases the risk of cardiovascular disease and type 2 diabetes mellitus. Here, we investigated the crosstalk between liver and adipocytes by characterizing EVs secreted by primary hepatocytes isolated from Zucker rat model, and studied the effect they have on 3T3-L1 adipocytes. We found that steatotic hepatocytes secrete EVs with significantly reduced exosomal markers in comparison with their lean counterpart. Moreover, proteomic analysis revealed that those EVs reflect the metabolic state of the parent cell in that the majority of proteins upregulated relate to fat metabolism, fatty acid synthesis, glycolysis, and pentose phosphate pathway. In addition, hepatocytes-secreted EVs influenced lipolysis and insulin sensitivity in recipient 3T3-L1 adipocytes. Untargeted metabolomic analysis detected alterations in different adipocyte metabolic pathways in cells treated with hepatic EVs. In summary, our work showed that steatosis has a significant impact in the amount and composition of EVs secreted by hepatocytes. Moreover, our data point to the involvement of hepatic-EVs in the development of pathologies associated with MetS. Inflamació Síndrome metabòlica Obesitat Inflammation Metabolic syndrome Obesity Extracellular vesicles released by steatotic hepatocytes alter adipocyte metabolism