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oai:recercat.cat:2445/1844482025-12-05T12:17:59Zcom_2072_1057col_2072_478916col_2072_478917

Comparative pangenome analysis of capsulated Haemophilus influenzae serotype f highlights their high genomic stability González Díaz, Aida Carrera Salinas, Anna Pinto, Miguel Cubero, Meritxell Ende, Arie van der Langereis, Jeroen D. Domínguez, M. Ángeles Ardanuy, Carmen Bajanca Lavado, Paula Marti, Sara Bacils Genòmica Bacillus (Bacteria) Genomics Haemophilus influenzae is an opportunistic pathogen adapted to the human respiratory tract. Non-typeable H. influenzae are highly heterogeneous, but few studies have analysed the genomic variability of capsulated strains. This study aims to examine the genetic diversity of 37 serotype f isolates from the Netherlands, Portugal, and Spain, and to compare all capsulated genomes available on public databases. Serotype f isolates belonged to CC124 and shared few single nucleotide polymorphisms (SNPs) (n = 10,999), but a high core genome (> 80%). Three main clades were identified by the presence of 75, 60 and 41 exclusive genes for each clade, respectively. Multi-locus sequence type analysis of all capsulated genomes revealed a reduced number of clonal complexes associated with each serotype. Pangenome analysis showed a large pool of genes (n = 6360), many of which were accessory genome (n = 5323). Phylogenetic analysis revealed that serotypes a, b, and f had greater diversity. The total number of SNPs in serotype f was significantly lower than in serotypes a, b, and e (p < 0.0001), indicating low variability within the serotype f clonal complexes. Capsulated H. influenzae are genetically homogeneous, with few lineages in each serotype. Serotype f has high genetic stability regardless of time and country of isolation. 2022-03-28T13:25:03Z 2022-03-28T13:25:03Z 2022-02-24 2022-03-25T09:54:56Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Reproducció del document publicat a: https://doi.org/10.1038/s41598-022-07185-5 Scientific Reports, 2022, vol 12, num 1 https://doi.org/10.1038/s41598-022-07185-5 cc by (c) Gonzalez Diaz, Aida et al, 2022 http://creativecommons.org/licenses/by/3.0/es/ info:eu-repo/semantics/openAccess Springer Science and Business Media LLC Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))