2026-04-13T02:38:48Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/1844482025-12-05T12:17:59Zcom_2072_1057col_2072_478916col_2072_478917

00925njm 22002777a 4500 dc González Díaz, Aida author Carrera Salinas, Anna author Pinto, Miguel author Cubero, Meritxell author Ende, Arie van der author Langereis, Jeroen D. author Domínguez, M. Ángeles author Ardanuy, Carmen author Bajanca Lavado, Paula author Marti, Sara author 2022-03-28T13:25:03Z 2022-03-28T13:25:03Z 2022-02-24 2022-03-25T09:54:56Z Haemophilus influenzae is an opportunistic pathogen adapted to the human respiratory tract. Non-typeable H. influenzae are highly heterogeneous, but few studies have analysed the genomic variability of capsulated strains. This study aims to examine the genetic diversity of 37 serotype f isolates from the Netherlands, Portugal, and Spain, and to compare all capsulated genomes available on public databases. Serotype f isolates belonged to CC124 and shared few single nucleotide polymorphisms (SNPs) (n = 10,999), but a high core genome (> 80%). Three main clades were identified by the presence of 75, 60 and 41 exclusive genes for each clade, respectively. Multi-locus sequence type analysis of all capsulated genomes revealed a reduced number of clonal complexes associated with each serotype. Pangenome analysis showed a large pool of genes (n = 6360), many of which were accessory genome (n = 5323). Phylogenetic analysis revealed that serotypes a, b, and f had greater diversity. The total number of SNPs in serotype f was significantly lower than in serotypes a, b, and e (p < 0.0001), indicating low variability within the serotype f clonal complexes. Capsulated H. influenzae are genetically homogeneous, with few lineages in each serotype. Serotype f has high genetic stability regardless of time and country of isolation. Bacils Genòmica Bacillus (Bacteria) Genomics Comparative pangenome analysis of capsulated Haemophilus influenzae serotype f highlights their high genomic stability