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oai:recercat.cat:2445/1844122025-12-04T21:27:09Zcom_2072_1057col_2072_478779col_2072_478917

Alkaloids Analysis of Habranthus cardenasianus (Amaryllidaceae), Anti-Cholinesterase Activity and Biomass Production by Propagation Strategies Zaragoza-Puchol, Daniel Ortiz, Javier E. Orden, Alejandro A. Sánchez, Marianela Palermo, Jorge Tapia, Alejandro Bastida Armengol, Jaume Feresin, Gabriela E. Amaril·lidàcies Alcaloides Compostos bioactius Amaryllidaceae Alkaloids Bioactive compounds Plants in the Amaryllidaceae family synthesize a diversity of bioactive alkaloids. Some of these plant species are not abundant and have a low natural multiplication rate. The aims of this work were the alkaloids analysis of a Habranthus cardenasianus bulbs extract, the evaluation of its inhibitory activity against cholinesterases, and to test several propagation strategies for biomass production. Eleven compounds were characterized by GC-MS in the alkaloid extract, which showed a relatively high proportion of tazettine. The known alkaloids tazettine, haemanthamine, and the epimer mixture haemanthidine/6-epi-haemanthidine were isolated and identified by spectroscopic methods. Inhibitory cholinesterases activity was not detected. Three forms of propagation were performed: bulb propagation from seed, cut-induced bulb division, and micropropagated bulbs. Finally, different imbibition and post-collection times were evaluated in seed germination assays. The best propagation method was cut-induced bulb division with longitudinal cuts into quarters (T1) while the best conditions for seed germination were 0-day of post-collection and two days of imbibition. The alkaloids analyses of the H. cardenasianus bulbs showed that they are a source of anti-tumoral alkaloids, especially pretazettine (tazettine) and T1 is a sustainable strategy for its propagation and domestication to produce bioactive alkaloids. Keywords: Amaryllidaceae; bioactive alkaloids; GC-MS; propagation methods; biomass production 2022-03-25T09:40:16Z 2022-03-25T09:40:16Z 2021 2022-03-25T09:40:16Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Reproducció del document publicat a: https://doi.org/10.3390/molecules26010192 Molecules, 2021, vol. 26, num. 1, p. 192 https://doi.org/10.3390/molecules26010192 cc-by (c) Zaragoza-Puchol, Daniel et al., 2021 https://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess MDPI Articles publicats en revistes (Biologia, Sanitat i Medi Ambient)