2026-04-13T02:06:05Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/1844122025-12-04T21:27:09Zcom_2072_1057col_2072_478779col_2072_478917

00925njm 22002777a 4500 dc Zaragoza-Puchol, Daniel author Ortiz, Javier E. author Orden, Alejandro A. author Sánchez, Marianela author Palermo, Jorge author Tapia, Alejandro author Bastida Armengol, Jaume author Feresin, Gabriela E. author 2022-03-25T09:40:16Z 2022-03-25T09:40:16Z 2021 2022-03-25T09:40:16Z Plants in the Amaryllidaceae family synthesize a diversity of bioactive alkaloids. Some of these plant species are not abundant and have a low natural multiplication rate. The aims of this work were the alkaloids analysis of a Habranthus cardenasianus bulbs extract, the evaluation of its inhibitory activity against cholinesterases, and to test several propagation strategies for biomass production. Eleven compounds were characterized by GC-MS in the alkaloid extract, which showed a relatively high proportion of tazettine. The known alkaloids tazettine, haemanthamine, and the epimer mixture haemanthidine/6-epi-haemanthidine were isolated and identified by spectroscopic methods. Inhibitory cholinesterases activity was not detected. Three forms of propagation were performed: bulb propagation from seed, cut-induced bulb division, and micropropagated bulbs. Finally, different imbibition and post-collection times were evaluated in seed germination assays. The best propagation method was cut-induced bulb division with longitudinal cuts into quarters (T1) while the best conditions for seed germination were 0-day of post-collection and two days of imbibition. The alkaloids analyses of the H. cardenasianus bulbs showed that they are a source of anti-tumoral alkaloids, especially pretazettine (tazettine) and T1 is a sustainable strategy for its propagation and domestication to produce bioactive alkaloids. Keywords: Amaryllidaceae; bioactive alkaloids; GC-MS; propagation methods; biomass production Amaril·lidàcies Alcaloides Compostos bioactius Amaryllidaceae Alkaloids Bioactive compounds Alkaloids Analysis of Habranthus cardenasianus (Amaryllidaceae), Anti-Cholinesterase Activity and Biomass Production by Propagation Strategies