2026-04-19T17:43:14Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/1835222025-12-04T19:31:55Zcom_2072_1057col_2072_478908col_2072_478917

00925njm 22002777a 4500 dc Calon, Alexandre author Espinet, Elisa author Palomo Ponce, Sergio author Tauriello, Daniele V. F. author Iglesias, Mar author Céspedes, María author Sevillano, Marta author Nadal, Cristina author Jung, Peter author Zhang, Xiang author Byrom, Daniel author Riera i Escalé, Antoni author Rossell, David author Mangues, Ramon author Massagué i Solé, Joan, 1953- author Sancho, Elena author Batlle, Eduard author 2022-02-24T19:00:17Z 2022-02-24T19:00:17Z 2014-05-05 2022-02-24T12:05:08Z Metastasis depends on a gene program expressed by the tumor microenvironment upon TGF-beta stimulation. CRC (Colorectal cancer) cell lines did not induce robust stromal TGF- beta responses when injected into nude mice as shown by lack of p- SMAD2 accumulation in tumor-associated stromal cells. To enforce high TGF-beta signaling in xenografts, we engineered CRC cell lines to secrete active TGF-beta. Subcutaneous tumors obtained from HT29-M6TGF-β, KM12L4aTGF-β cells and SW48TGF-β cells contained abundant p-SMAD2+ stromal cells. Càncer colorectal Metàstasi Expressió gènica Colorectal cancer Metastasis Gene expression Immunostaining Protocol: P-Smad2 (Xenograft and Mice)