2026-04-18T00:16:19Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/1834672025-12-04T19:31:25Zcom_2072_1057col_2072_478908col_2072_478917

Immunostaining Protocol: P-Stat3 (Xenograft and Mice) Calon, Alexandre Espinet, Elisa Palomo Ponce, Sergio Tauriello, Daniele V. F. Iglesias, Mar Cespedes, María Virtudes Sevillano, Marta Nadal, Cristina Jung, Peter Zhang, Xiang H. F. Byrom, Daniel Riera i Escalé, Antoni Rossell, David Mangues, Ramon Massagué i Solé, Joan, 1953- Sancho, Elena Batlle, Eduard Càncer colorectal Metàstasi Expressió gènica Colorectal cancer Metastasis Gene expression We sought to understand the mechanisms behind the potent effect of stromal TGF-beta program on the capacity of colorectal cancer (CRC) cells to initiate metastasis. We discovered that mice subcutaneous tumors and metastases generated in the context of a TGF-beta activated microenvironment displayed prominent accumulation of p-STAT3 in CRC cells compared with those derived from control cells. STAT3 signaling depended on GP130 as shown by strong reduction of epithelial p STAT3 levels upon GP130 shRNA-mediated knockdown in CRC cells. 2022-02-24T19:00:57Z 2022-02-24T19:00:57Z 2014-03-05 2022-02-24T12:05:43Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2331-8325 https://hdl.handle.net/2445/183467 6544612 eng Reproducció del document publicat a: https://doi.org/10.21769/BioProtoc.1120 Bio-protocol, 2014, vol. 4, num. 9, p. e1120 https://doi.org/10.21769/BioProtoc.1120 (c) Bio-protocol LLC, 2014 info:eu-repo/semantics/openAccess 4 p. application/pdf Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona))