2026-04-17T20:09:42Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/1830162025-12-04T19:32:57Zcom_2072_1057col_2072_478908col_2072_478917

00925njm 22002777a 4500 dc Martin Malpartida, Pau author Hausvik, Emil author Underhaug, Jarl author Torner, Carles author Martinez, Aurora author Macias, Maria Jesus author 2022-02-08T17:44:05Z 2022-11-19T06:10:26Z 2021-11-19 2022-02-08T13:49:54Z The identification of new drugs for novel therapeutic targets requires the screening of libraries containing tens of thousands of compounds. While experimental screenings are assisted by high-throughput technologies, in target-based biophysical assays, such as differential scanning fluorimetry (DSF), the analysis steps must be calculated manually, often combining several software packages. To simplify the determination of the melting temperature (Tm) of the target and the change induced by ligand binding (?Tm), we developed the HTSDSF explorer, a versatile, all-in-one, user-friendly application suite. Implemented as a server-client application, in the primary screenings, HTSDSF explorer pre-analyzes and displays the Tm and ?Tm results interactively, thereby allowing the user to study hundreds of conditions and select the primary hits in minutes. This application also allows the determination of preliminary binding constants (KD) through a series of subsequent dose–response assays on the primary hits, thereby facilitating the ranking of validated hits and the advance of drug discovery efforts. © 2021 Elsevier Ltd automatic Tm automatic KD analysis Differential scanning fluorimetry high-throughput compound screening thermal shift assay Arquitectura client-servidor (Informàtica) Medicaments Client/server computing Drugs HTSDSF Explorer, A Novel Tool to Analyze High-throughput DSF Screenings: HTSDSF Explorer, a tool for DSF screenings