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Synthesis, RNAi activity and nuclease-resistant properties of apolar carbohydrates siRNA conjugates Vengut Climent, Empar Terrazas Martínez, Montserrat Lucas, Ricardo Arévalo Ruiz, Matilde Eritja i Casadellà, Ramon Morales, Juan Carlos RNA Oligonucleòtids Química orgànica RNA Oligonucleotides Organic chemistry Oligoribonucleotide conjugates carrying apolar carbohydrates at the 5′-end and the corresponding siRNA duplexes have been prepared using phosphoramidite chemistry. All the carbohydrate-siRNA derivatives were compatible with RNA interference machinery if transfected with oligofectamine. In the absence of a transfection agent, some of them exerted certain reduction of gene expression. Double-tailed permethylated glucose conjugated to siRNA through a long spacer inhibited gene expression up to 26% compared to the scrambled duplex. Such modifications contribute positively to the stability of oligoribonucleotides against 5′-exonuclease degradation. 2021-08-30T12:45:07Z 2021-08-30T12:45:07Z 2013-07-15 2021-08-30T12:45:07Z info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion Versió postprint del document publicat a: https://doi.org/10.1016/j.bmcl.2013.05.065 Bioorganic & Medicinal Chemistry Letters, 2013, vol. 23, num. 14, p. 4048-4051 https://doi.org/10.1016/j.bmcl.2013.05.065 (c) Elsevier Ltd, 2013 info:eu-repo/semantics/openAccess Elsevier Ltd Articles publicats en revistes (Química Inorgànica i Orgànica)