2026-04-14T02:38:37Zhttps://recercat.cat/oai/request
oai:recercat.cat:2445/1797762025-12-04T21:45:24Zcom_2072_1057col_2072_478917col_2072_478933
RECERCAT author Vengut Climent, Empar author Terrazas Martínez, Montserrat author Lucas, Ricardo author Arévalo Ruiz, Matilde author Eritja i Casadellà, Ramon author Morales, Juan Carlos 2021-08-30T12:45:07Z2021-08-30T12:45:07Z2013-07-152021-08-30T12:45:07Z Oligoribonucleotide conjugates carrying apolar carbohydrates at the 5′-end and the corresponding siRNA duplexes have been prepared using phosphoramidite chemistry. All the carbohydrate-siRNA derivatives were compatible with RNA interference machinery if transfected with oligofectamine. In the absence of a transfection agent, some of them exerted certain reduction of gene expression. Double-tailed permethylated glucose conjugated to siRNA through a long spacer inhibited gene expression up to 26% compared to the scrambled duplex. Such modifications contribute positively to the stability of oligoribonucleotides against 5′-exonuclease degradation. (c) Elsevier Ltd, 2013 info:eu-repo/semantics/openAccess RNA Oligonucleòtids Química orgànica RNA Oligonucleotides Organic chemistry Synthesis, RNAi activity and nuclease-resistant properties of apolar carbohydrates siRNA conjugates info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion