2026-04-14T04:55:30Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/1797762025-12-04T21:45:24Zcom_2072_1057col_2072_478917col_2072_478933

00925njm 22002777a 4500 dc Vengut Climent, Empar author Terrazas Martínez, Montserrat author Lucas, Ricardo author Arévalo Ruiz, Matilde author Eritja i Casadellà, Ramon author Morales, Juan Carlos author 2021-08-30T12:45:07Z 2021-08-30T12:45:07Z 2013-07-15 2021-08-30T12:45:07Z Oligoribonucleotide conjugates carrying apolar carbohydrates at the 5′-end and the corresponding siRNA duplexes have been prepared using phosphoramidite chemistry. All the carbohydrate-siRNA derivatives were compatible with RNA interference machinery if transfected with oligofectamine. In the absence of a transfection agent, some of them exerted certain reduction of gene expression. Double-tailed permethylated glucose conjugated to siRNA through a long spacer inhibited gene expression up to 26% compared to the scrambled duplex. Such modifications contribute positively to the stability of oligoribonucleotides against 5′-exonuclease degradation. RNA Oligonucleòtids Química orgànica RNA Oligonucleotides Organic chemistry Synthesis, RNAi activity and nuclease-resistant properties of apolar carbohydrates siRNA conjugates