2026-04-18T07:09:12Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/1793072025-11-20T13:51:20Zcom_2072_1057col_2072_478799col_2072_478917

Sirtuin 1 regulation of developmental genes during differentiation of stem cells Calvanese, Vincenzo Lara, Ester Suárez-Alvarez, Beatriz Abu Dawud, Raed Vázquez-Chantada, Mercedes Martínez Chantar, Maria Luz Embade, Nieves López Nieva, Pilar Horrillo, Angelica Hmadcha, Abdelkrim Soria Cubero, Berta Piazzolla, Daniella Herranz, Daniela Serrano Marugán, Manuel Mato, José M. Andrew, Peter W. López Larrea, Carlos Esteller, Manel Fraga, Mario F. Diferenciació cel·lular Cèl·lules mare embrionàries Expressió gènica Cell diferentiation Embryonic stem cells Gene expression The longevity-promoting NAD+-dependent class III histone deacetylase Sirtuin 1 (SIRT1) is involved in stem cell function by controlling cell fate decision and/or by regulating the p53-dependent expression of NANOG. We show that SIRT1 is down-regulated precisely during human embryonic stem cell differentiation at both mRNA and protein levels and that the decrease in Sirt1 mRNA is mediated by a molecular pathway that involves the RNA-binding protein HuR and the arginine methyltransferase coactivator-associated arginine methyltransferase 1 (CARM1). SIRT1 down-regulation leads to reactivation of key developmental genes such as the neuroretinal morphogenesis effectors DLL4, TBX3, and PAX6, which are epigenetically repressed by this histone deacetylase in pluripotent human embryonic stem cells. Our results indicate that SIRT1 is regulated during stem cell differentiation in the context of a yet-unknown epigenetic pathway that controls specific developmental genes in embryonic stem cells. 2021-07-22T12:24:28Z 2021-07-22T12:24:28Z 2010-08-03 2021-07-22T12:24:28Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 0027-8424 https://hdl.handle.net/2445/179307 700240 20631301 eng Reproducció del document publicat a: https://doi.org/10.1073/pnas.1001399107 Proceedings of the National Academy of Sciences of the United States of America - PNAS, 2010, vol. 107, num. 31, p. 13736-13741 https://doi.org/10.1073/pnas.1001399107 (c) Calvanese, Vincenzo et al., 2010 info:eu-repo/semantics/openAccess 6 p. application/pdf application/pdf National Academy of Sciences Articles publicats en revistes (Ciències Fisiològiques)