2026-04-14T06:32:33Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/1788882025-12-04T22:56:38Zcom_2072_1057col_2072_478782col_2072_478917

Parallel Clamps and Polypurine Hairpins (PPRH) for Gene Silencing and Triplex-Affinity Capture: Design, Synthesis, and Use Aviñó Andrés, Anna Eritja i Casadellà, Ramon Ciudad i Gómez, Carlos Julián Noé Mata, Verónica ADN Teràpia genètica Reparació de l'ADN DNA Gene therapy DNA repair Nucleic acid triplexes are formed when a DNA or RNA oligonucleotide binds to a polypurine-polypyrimidine-rich sequence. Triplexes have wide therapeutic applications such as gene silencing or site-specific mutagenesis. In addition, protocols based on triplex-affinity capture have been used for detecting nucleic acids in biosensing platforms. In this article, the design, synthesis, and use of parallel clamps and polypurine-reversed hairpins (PPRH) to bind to target polypyrimidine targets are described. The combination of the polypurine Watson-Crick strand with the triplex-forming strand in a single molecule produces highly stable triplexes allowing targeting of single- and double-stranded nucleic acid sequences. On the other hand, PPRHs are easily prepared and work at nanomolar range, like siRNAs, and at a lower concentration than that needed for antisense ODNs or TFOs. However, the stability of PPRHs is higher than that of siRNAs. In addition, PPRHs circumvent off-target effects and are non-immunogenic. 2021-07-08T10:32:28Z 2021-07-08T10:32:28Z 2019-03-26 2021-07-08T10:32:28Z info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion 1934-9289 https://hdl.handle.net/2445/178888 690751 eng Versió postprint del document publicat a: https://doi.org/10.1002/cpnc.78 Current protocols in nucleic acid chemistry, 2019, vol. 77, num. 1, p. e78 https://doi.org/10.1002/cpnc.78 (c) John Wiley & Sons, 2019 info:eu-repo/semantics/openAccess application/pdf John Wiley & Sons Articles publicats en revistes (Bioquímica i Fisiologia)