2026-04-13T05:57:08Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/1788882025-12-04T22:56:38Zcom_2072_1057col_2072_478782col_2072_478917

00925njm 22002777a 4500 dc Aviñó Andrés, Anna author Eritja i Casadellà, Ramon author Ciudad i Gómez, Carlos Julián author Noé Mata, Verónica author 2021-07-08T10:32:28Z 2021-07-08T10:32:28Z 2019-03-26 2021-07-08T10:32:28Z Nucleic acid triplexes are formed when a DNA or RNA oligonucleotide binds to a polypurine-polypyrimidine-rich sequence. Triplexes have wide therapeutic applications such as gene silencing or site-specific mutagenesis. In addition, protocols based on triplex-affinity capture have been used for detecting nucleic acids in biosensing platforms. In this article, the design, synthesis, and use of parallel clamps and polypurine-reversed hairpins (PPRH) to bind to target polypyrimidine targets are described. The combination of the polypurine Watson-Crick strand with the triplex-forming strand in a single molecule produces highly stable triplexes allowing targeting of single- and double-stranded nucleic acid sequences. On the other hand, PPRHs are easily prepared and work at nanomolar range, like siRNAs, and at a lower concentration than that needed for antisense ODNs or TFOs. However, the stability of PPRHs is higher than that of siRNAs. In addition, PPRHs circumvent off-target effects and are non-immunogenic. ADN Teràpia genètica Reparació de l'ADN DNA Gene therapy DNA repair Parallel Clamps and Polypurine Hairpins (PPRH) for Gene Silencing and Triplex-Affinity Capture: Design, Synthesis, and Use