2026-04-13T00:08:08Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/1781122026-02-08T00:30:03Zcom_2072_1057col_2072_478902col_2072_478917

00925njm 22002777a 4500 dc Barbero Castillo, Almudena author Riefolo, Fabio author Matera, Carlo author Caldas Martínez, Sara author Mateos Aparicio, Pedro author Weinert, Julia F. author Garrido Charles, Aida author Claro Izaguirre, Enrique author Sánchez Vives, María Victoria author Gorostiza Langa, Pablo Ignacio author 2021-06-08T08:13:03Z 2021-06-08T08:13:03Z 2021-05-21 Reproducció del document publicat a:https://doi.org/10.1002/advs.202005027 The ability to control neural activity is essential for research not only in basic neuroscience, as spatiotemporal control of activity is a fundamental experimental tool, but also in clinical neurology for therapeutic brain interventions. Transcranial-magnetic, ultrasound, and alternating/direct current (AC/DC) stimulation are some available means of spatiotemporal controlled neuromodulation. There is also light-mediated control, such as optogenetics, which has revolutionized neuroscience research, yet its clinical translation is hampered by the need for gene manipulation. As a drug-based light-mediated control, the effect of a photoswitchable muscarinic agonist (Phthalimide-Azo-Iper (PAI)) on a brain network is evaluated in this study. First, the conditions to manipulate M2 muscarinic receptors with light in the experimental setup are determined. Next, physiological synchronous emergent cortical activity consisting of slow oscillations—as in slow wave sleep—is transformed into a higher frequency pattern in the cerebral cortex, both in vitro and in vivo, as a consequence of PAI activation with light. These results open the way to study cholinergic neuromodulation and to control spatiotemporal patterns of activity in different brain states, their transitions, and their links to cognition and behavior. The approach can be applied to different organisms and does not require genetic manipulation, which would make it translational to humans. Neurociències Neurologia Neurosciences Neurology Control of Brain State Transitions with a Photoswitchable Muscarinic Agonist