2026-04-17T20:35:57Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/1779242025-12-05T14:51:23Zcom_2072_1057col_2072_478917col_2072_478924

00925njm 22002777a 4500 dc Mazzolini, Rocco author González, Nuria author Garcia-Garijo, Andrea author Millanes-Romero, Alba author Peiró, Sandra author Smith, Susan author García de Herreros, Antonio author Canudas Puig, Sílvia author 2021-06-03T11:42:22Z 2021-06-03T11:42:22Z 2018-01-09 2021-06-03T11:42:22Z Besides controlling epithelial-to-mesenchymal transition (EMT) and cell invasion, the Snail1 transcriptional factor also provides cells with cancer stem cell features. Since telomere maintenance is essential for stemness, we have examined the control of telomere integrity by Snail1. Fluorescence in situ hybridization (FISH) analysis indicates that Snail1-depleted mouse mesenchymal stem cells (MSC) have both a dramatic increase of telomere alterations and shorter telomeres. Remarkably, Snail1-deficient MSC present higher levels of both telomerase activity and the long non-coding RNA called telomeric repeat-containing RNA (TERRA), an RNA that controls telomere integrity. Accordingly, Snail1 expression downregulates expression of the telomerase gene (TERT) as well as of TERRA 2q, 11q and 18q. TERRA and TERT are transiently downregulated during TGF-induced EMT in NMuMG cells, correlating with Snail1 expression. Global transcriptome analysis indicates that ectopic expression of TERRA affects the transcription of some genes induced during EMT, such as fibronectin, whereas that of TERT does not modify those genes. We propose that Snail1 repression of TERRA is required not only for telomere maintenance but also for the expression of a subset of mesenchymal genes. Cèl·lules mare Telòmer Càncer Stem cells Telomere Cancer Snail1 transcription factor controls telomere transcription and integrity