2026-04-17T06:40:35Zhttps://recercat.cat/oai/request
oai:recercat.cat:2445/1771272025-12-05T09:24:39Zcom_2072_1057col_2072_478781col_2072_478799col_2072_478917
RECERCAT author Muñoz Moruno, Purificación author Mora Fayos, Sílvia author Sevilla, Lidia author Kaliman, Perla author Tomàs, Eva author Gumà i Garcia, Anna Maria author Testar, Xavier author Palacín Prieto, Manuel author Zorzano Olarte, Antonio 2021-05-10T14:18:24Z2021-05-10T14:18:24Z1996-04-052021-05-10T14:18:24Z Caveolin is believed to play an important role in sorting processes, vesicular trafficking, transmembrane signaling, and molecular transport across membranes. In this study we have evaluated the expression and distribution of caveolin in skeletal muscle and its interaction with GLUT4 glucose carriers. Caveolin was expressed to substantial levels in muscle and its expression was regulated in muscle; aging and high fat diet enhanced caveolin expression in skeletal muscle and inversely, myogenesis down-regulated caveolin in L6E9 cells. Under fasting conditions, most of caveolin was found in intracellular membranes and the caveolin present in the cell surface was found in both sarcolemma and T-tubules. Insulin administration led to a redistribution of caveolin from intracellular high density membrane fractions to intracellular lighter density fractions and to the cell surface; this pattern of insulin-induced redistribution was different to what was shown by GLUT4. These results suggests that caveolin is a component of an insulin-regulated machinery of vesicular transport in muscle. Quantitative immunoisolation of GLUT4 vesicles obtained from different intracellular GLUT4 populations revealed the absence of caveolin which substantiates the lack of colocalization of intracellular GLUT4 and caveolin. This indicates that caveolin is not involved in intracellular GLUT4 trafficking in skeletal muscle. (c) American Society for Biochemistry and Molecular Biology, 1996 info:eu-repo/semantics/openAccess Insulina Fisiologia Proteïnes de membrana Músculs Proteïnes Insulin Physiology Membrane proteins Muscles Proteins Expression and insulin-regulated distribution of caveolin in skeletal muscle. Caveolin does not colocalize with GLUT4 in intracellular membranes info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion