2026-04-17T04:25:07Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/1767972026-03-07T19:43:46Zcom_2072_1057col_2072_478781col_2072_478917

00925njm 22002777a 4500 dc Xu, Xiaojun author Gopalacharyullu, Peddinti author Seppänen-Laakso, Tuulikki author Ruskeepaa, Anna-Liisa author Aye, Cho-Cho author Carson, Brian P. author Mora Fayos, Sílvia author Oresic, Matej author Teleman, Aurelio A. author 2021-04-27T15:19:29Z 2021-04-27T15:19:29Z 2012-01-19 2021-04-27T15:19:30Z The insulin/IGF signaling pathway is a highly conserved regulator of metabolism in flies and mammals, regulating multiple physiological functions including lipid metabolism. Although insulin signaling is known to regulate the activity of a number of enzymes in metabolic pathways, a comprehensive understanding of how the insulin signaling pathway regulates metabolic pathways is still lacking. Accepted knowledge suggests the key regulated step in triglyceride (TAG) catabolism is the release of fatty acids from TAG via the action of lipases. We show here that an additional, important regulated step is the activation of fatty acids for beta-oxidation via Acyl Co-A synthetases (ACS). We identify pudgy as an ACS that is transcriptionally regulated by direct FOXO action in Drosophila. Increasing or reducing pudgy expression in vivo causes a decrease or increase in organismal TAG levels respectively, indicating that pudgy expression levels are important for proper lipid homeostasis. We show that multiple ACSs are also transcriptionally regulated by insulin signaling in mammalian cells. In sum, we identify fatty acid activation onto CoA as an important, regulated step in triglyceride catabolism, and we identify a mechanistic link through which insulin regulates lipid homeostasis. Insulina Teixit adipós Insulin Adipose tissues Insulin signalling regulates fatty acid catabolisme at the level of CoA activation