2026-04-17T03:33:06Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/1761062026-02-20T06:13:34Zcom_2072_1057col_2072_478780col_2072_478905col_2072_478917

00925njm 22002777a 4500 dc Carvajal Romero, Silvia author Perramón Corominas, Meritxell author Casals Mercadal, Gregori author Oró Bozzini, Denise author Ribera Sabaté, Jordi author Morales Ruiz, Manuel author Casals, Eudald author Casado, Pedro author Melgar-Lesmes, Pedro author Fernández Varo, Guillermo author Cutillas, Pedro R. author Puntes, Víctor author Jiménez Povedano, Wladimiro author 2021-04-09T09:43:28Z 2021-04-09T09:43:28Z 2019-11-27 2021-04-09T09:43:28Z Cerium oxide nanoparticles (CeO2NPs) possess powerful antioxidant properties, thus emerging as a potential therapeutic tool in non-alcoholic fatty liver disease (NAFLD) progression, which is characterized by a high presence of reactive oxygen species (ROS). The aim of this study was to elucidate whether CeO2NPs can prevent or attenuate oxidant injury in the hepatic human cell line HepG2 and to investigate the mechanisms involved in this phenomenon. The effect of CeO2NPs on cell viability and ROS scavenging was determined, the differential expression of pro-inflammatory and oxidative stress-related genes was analyzed, and a proteomic analysis was performed to assess the impact of CeO2NPs on cell phosphorylation in human hepatic cells under oxidative stress conditions. CeO2NPs did not modify HepG2 cell viability in basal conditions but reduced H2O2- and lipopolysaccharide (LPS)-induced cell death and prevented H2O2-induced overexpression of MPO, PTGS1 and iNOS. Phosphoproteomic analysis showed that CeO2NPs reverted the H2O2-mediated increase in the phosphorylation of peptides related to cellular proliferation, stress response, and gene transcription regulation, and interfered with H2O2 effects on mTOR, MAPK/ERK, CK2A1 and PKACA signaling pathways. In conclusion, CeO2NPs protect HepG2 cells from cell-induced oxidative damage, reducing ROS generation and inflammatory gene expression as well as regulation of kinase-driven cell survival pathways. Ceri (Element químic) Estrès oxidatiu Cèl·lules hepàtiques Cerium Oxidative stress Liver cells Cerium Oxide Nanoparticles Protect against Oxidant Injury and Interfere with Oxidative Mediated Kinase Signaling in Human-Derived Hepatocytes