2026-04-17T13:53:57Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/1750002025-12-05T09:41:36Zcom_2072_1057col_2072_478778col_2072_478909col_2072_478917

00925njm 22002777a 4500 dc Monastiri, Abir author Mechri, Badereddine author Vázquez-González, Ana author Couilh, Meriadeg Ar author Chakroun, Mohamed author Loussaief, Chawki author Mastouri, Maha author Dimassi, Najet author Boughzala, Lamjed author Aouni, Mahjoub author Serra Cobo, Jordi author 2021-03-12T17:24:56Z 2021-03-12T17:24:56Z 2018 2021-03-12T17:24:56Z A West Nile virus (WNV) outbreak occurred in Tunisia between mid-July and December 2012. To assess the epidemiological features of the WNV transmission cycle, human cerebrospinal fluid samples from patients with suspected cases (n = 79), Culex pipiens mosquitoes (n = 583) and serum specimens from domestic and migratory birds (n = 70) were collected for 4 years (2011-2014) in the Tunisian Sahel region. Viral testing was performed by polymerase chain reaction (PCR). The WNV genome was detected in 7 patients (8.8%), 4 Culex pipiens pools, and a domestic mallard (Anas platyrhynchos). All PCR-positive samples were from the Monastir region. Phylogenetic analysis revealed that two different WNV strain groups circulated, and isolates from the reservoir (bird), vector (Culex pipiens), and dead-end hosts (humans) were closely related. The Monastir region is a hot-spot for WNV infection, and the reiterative presence of WNV over the years has increased the risk of viral reemergence in Tunisia, which highlights the need for more enhanced and effective WNV surveillance in humans with public awareness campaigns strengthened by monitoring mosquitoes and maintaining avian surveillance for early detection of WNV circulation. Virus Tunísia Epidemiologia Viruses Tunisia Epidemiology A four-year survey (2011-2014) of West Nile virus infection in humans, mosquitoes and birds, including the 2012 meningoencephalitis outbreak in Tunisia