2026-04-13T09:46:11Zhttps://recercat.cat/oai/request

oai:recercat.cat:2445/1742722025-11-19T20:55:20Zcom_2072_1057col_2072_478917col_2072_478933

00925njm 22002777a 4500 dc Mendive Tapia, Lorena author Zhao, Can author Akram, Ahsan R. author Preciado Gallego, Sara author Albericio Palomera, Fernando author Lee, Martin author Serrels, Alan author Kielland, Nicola author Read, Nick D. author Lavilla Grífols, Rodolfo author Vendrell, Marc author 2021-02-24T16:03:09Z 2021-02-24T16:03:09Z 2016-03-09 2021-02-24T16:03:09Z Fluorescent antimicrobial peptides are promising structures for in situ, real-time imaging of fungal infection. Here we report a fluorogenic probe to image Aspergillus fumigatus directly in human pulmonary tissue. We have developed a fluorogenic Trp-BODIPY amino acid with a spacer-free C-C linkage between Trp and a BODIPY fluorogen, which shows remarkable fluorescence enhancement in hydrophobic microenvironments. The incorporation of our fluorogenic amino acid in short antimicrobial peptides does not impair their selectivity for fungal cells, and enables rapid and direct fungal imaging without any washing steps. We have optimized the stability of our probes in human samples to perform multi-photon imaging of A. fumigatus in ex vivo human tissue. The incorporation of our unique BODIPY fluorogen in biologically relevant peptides will accelerate the development of novel imaging probes with high sensitivity and specificity. Pèptids Micosi Aspergillus parasiticus Aminoàcids Peptides Mycosis Aspergillus parasiticus Amino acids Spacer-free BODIPY fluorogens in antimicrobial peptides for direct imaging of fungal infection in human tissue