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Ancestral function ofInhibitors-of-kappaB regulates Caenorhabditis elegans development Brena, David Bertran, Joan Porta de la Riva, Montserrat Guillen, Yolanda Cornes, Eric Kukhtar, Dmytro Campos Vicens, Lluís Fernández, Lierni Pecharroman, Irene García López, Albert Islam, Abul B. M. M. K. Marruecos, Laura Bigas Salvans, Anna Cerón Madrigal, Julián Espinosa, Lluís Anàlisi de proteïnes Nematodes Analysis of proteins Nematodes Mammalian I kappa B proteins (I kappa Bs) exert their main function as negative regulators of NF-kappa B, a central signaling pathway controlling immunity and inflammation. An alternative chromatin role for I kappa Bs has been shown to affect stemness and cell differentiation. However, the involvement of NF-kappa B in this function has not been excluded. NFKI-1 and IKB-1 are I kappa B homologs in Caenorhabditis elegans, which lacks NF-kappa B nuclear effectors. We found that nfki-1 and ikb-1 mutants display developmental defects that phenocopy mutations in Polycomb and UTX-1 histone demethylase, suggesting a role for C. elegans I kappa Bs in chromatin regulation. Further supporting this possibility (1) we detected NFKI-1 in the nucleus of cells; (2) NFKI-1 and IKB-1 bind to histones and Polycomb proteins, (3) and associate with chromatin in vivo, and (4) mutations in nfki-1 and ikb-1 alter chromatin marks. Based on these results, we propose that ancestral I kappa B inhibitors modulate Polycomb activity at specific gene subsets with an impact on development. 2021-02-23T18:43:58Z 2021-02-23T18:43:58Z 2020-09-30 2021-02-08T10:33:13Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://hdl.handle.net/2445/174233 32999373 eng Reproducció del document publicat a: https://doi.org/10.1038/s41598-020-73146-5 Scientific Reports, 2020, vol. 10 https://doi.org/10.1038/s41598-020-73146-5 cc by (c) Brena et al., 2020 http://creativecommons.org/licenses/by/3.0/es/ info:eu-repo/semantics/openAccess 13 p. application/pdf application/pdf Nature Research Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))